ABSTRACT
OBJECTIVES: Chronic periodontitis is a common inflammatory disease of the oral cavity that causes destruction of periodontal tissues and bone around the teeth. Sclerostin is a protein encoded by the SOST gene. In this study, gingival crevicular fluid (GCF) levels of sclerostin in patients with chronic periodontitis were compared with those of healthy subjects. MATERIALS AND METHODS: In this case-control study, a total of 40 subjects were enrolled and divided into the healthy group (n=23) and chronic periodontitis group (n=17). GCF samples were collected, and the concentration of sclerostin was evaluated using enzyme-linked immunosorbent assay. Comparison of significance between groups was assessed using Mann-Whitney U test. RESULTS: Sclerostin concentration was significantly higher in the chronic periodontitis group compared with the healthy group (P < 0.005). CONCLUSION: Despite the limitations of this study, sclerostin can be a possible marker for assessment of periodontal health status.
Subject(s)
Humans , Case-Control Studies , Chronic Periodontitis , Enzyme-Linked Immunosorbent Assay , Gingival Crevicular Fluid , Healthy Volunteers , Mouth , Periodontitis , ToothABSTRACT
Both the length of extra-alveolar time and type of storage media are significant factors that can affect the long-term prognosis of replanted teeth. This study aims to compare propolis 50%, propolis 10%, Hank's balanced salt solution [HBSS], milk and egg white on periodontal ligament [PDL] cell survival for different time points. In this in vitro experimental study, we divided 60 extracted teeth without any periodontal diseases into five experimental and two control groups that consisted each experimental group with 10 and each control group with 5 teeth. The storage times were one and three hours for each media. The controls corresponded to 0-minute [positive] and 12-hour [negative] dry time. Rinsing in the experimental media, the teeth were treated with dispase and collagenase for one hour. Cell viability was determined by using trypan blue exclusion. Statistical analysis of the data was accomplished by using two-way analysis of variance [ANOVA] complemented by the Tukey's HSD post-hoc. Within one hour, there was no significant difference between the two propolis groups, however these two groups had significantly more viable PDL cells compared to the other experimental media [p<0.05]. The results of the three-hour group showed that propolis 10% was significantly better than egg white, whereas both propolis 10% and 50% were significantly better than milk [p<0.05]. Based on PDL cell viability, propolis could be recommended as a suitable biological storage media for avulsed teeth
ABSTRACT
Hydroxyapatite [Ca[10][PO][4][6][OH][2] is the major inorganic component of hard tissues, the best bio-active materials, which is compatible with the bone tissue. In addition, hydroxyapatite nanoparticles [Nano-HA] have received enormous national attention in medical and dental applications recently; but the ultimate fate of the Nano-HA within the body is still unknown. Degradation products of nanomaterials are potentially cytotoxic. Thus, it is essential to assess biocompatibility before their usage in clinical applications. Purpose of this research is to evaluate toxicity of hydroxyapatite nano particles on the human peripheral blood mononuclear cells. In this experimental study, nano sized, rod-like hydroxyapatite particles sterilizalied then HPBMCs were cultured on 96-well plate. Cells were exposed to Nano-HA at the following concentrations: 15.5, 32, 65, 125, 250, 500, 1000, 2000, 4000, 8000 ppm. Later, For measuring the cell vitality, MTT method was utilized .Measuring the photo absorption was done by ELISA READER system at 570 nm, which evaluated the vitality of cell by the value of MTT absorption cells. The statistical ANOVA test was used in this study. All of drug concentration were effective in Loweriy cellular biologic ectivity but none of them were statis fically significant. Therefore as a conclusion we can adjudicate that [Nano-HA] biomaterial is the material which is compatibility with the human blood mononuclear cells
ABSTRACT
Background and Aim: Antimicrobial effects of nano silver particles have received enormous attention in medical and dental applications. Despite of wide spread use of nanosilver there is not enough studies about its side effects on human. Some studies have exhibited cytotoxicity of nano-silver particles. This research was designed to investigate the cytotoxic effect of nano silver particles on L929 fibroblasts cells, by MTT assay
Materials and Methods: In this experimental study, nano silver particles with 5,10,20,30,40 and 50 ppm concentration were exposed to 10.000 L929 fibroblast cells then cell vitality was assessed after 2,24,48 and 72 hours by MTT method. The light absorption was evaluated by Elisa Reader and recorded. Data was subjected to ANOVA test for statistical analysis
Results: In all concentration groups, vitality of cells were the least after 24 hours, but the vitality of cells increased after 48 hours. The increase after 48H was statistically significant [p<0.05]. Also concentrations more than 20 ppm in 2,24 and 48 H were significantly cytotoxic for fibroblasts
Conclusion: Nano silver particles at concentrations less than 20 ppm after 72 hours did not have toxic effects
ABSTRACT
The purpose of this ex vivo study was to assess the effect of two root-end filling materials against Candida [C] albicans. ProRoot MTA and CEM Cement were compared immediately and 24 h after mixing, in two different concentrations [50 and 100 mg/mL]. A total of 50 culture wells were used and divided into experimental [n=10] and control groups [n=5]. Those with no medication served as positive and without C. albicans served as negative controls. All plates were incubated at 37 C. after 1, 24, and 48hours. At each interval, the presence of C. albicans was assessed and recorded by an independent observer. In addition to observing turbidity, 0.02 mL of samples from each cell was re-cultured on sabouraud dextrose agar plates to confirm change in fungal growth. The data were evaluated and analyzed using Kruskal-Wallis test. Although all fresh and set samples with experimental concentrations showed fungal growth after 1 h; they demonstrated complete fungicidal activity at 24 and 48-h time intervals. Under the conditions of this ex vivo study, CEM cement as well as ProRoot MTA has fungicidal effects against C. albicans even in concentration of 50 mg/mL and after 24 hours